Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb (A1021)

Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb (A1021)

Western blot analysis of various lysates, using Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb (A1021) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb
Catalog No. A1021
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. This gene is one in a cluster of serpin genes located on the q arm of chromosome 14. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human Alpha-1 Antichymotrypsin (Alpha-1 Antichymotrypsin (SERPINA3)) (NP_001076.2).
Sequence HPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA
Gene ID 12
Swiss prot P01011
Synonyms ACT; AACT; GIG24; GIG25; Alpha-1 Antichymotrypsin (SERPINA3)
Calculated MW 48kDa
Observed MW 50-76kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples MCF7, U2OS
Cellular location Secreted
Customer validation

WB (Homo sapiens, Mus musculus)

ELISA (Mus musculus)

IHC (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb images

ABclonal:Western blot - Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb (A1021)}

Western blot - Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb (A1021)

Western blot analysis of various lysates, using Alpha-1 Antichymotrypsin (SERPINA3) Rabbit pAb (A1021) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SERPINA3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SERPINA3. (Distance between topics and target gene indicate popularity.) SERPINA3

* Data provided by citexs.com, for reference only.

Publishing research using A1021? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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