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SELP Rabbit pAb (A12501)

Publication (1) Datasheet

ABclonal:Western blot - SELP Polyclonal Antibody (A12501)
ABclonal:Immunohistochemistry - SELP Polyclonal Antibody (A12501)


Product nameSELP Rabbit pAb
Catalog No.A12501
Host speciesRabbit
Purification methodAffinity purification
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 220-319 of human SELP (NP_002996.2).
Gene ID6403
Swiss protP16109
Calculated MW90kDa
Observed MW91kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse kidney
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - SELP Polyclonal Antibody (A12501) }

Western blot - SELP Polyclonal Antibody (A12501)

Western blot analysis of extracts of mouse kidney, using SELP Antibody (A12501) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - SELP Polyclonal Antibody (A12501) }

Immunohistochemistry - SELP Polyclonal Antibody (A12501)

Immunohistochemistry of paraffin-embedded human esophagus using SELP antibody (A12501) (40x lens).

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