Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Publications (4) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:SARS-CoV-2

ABclonal:Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2,using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Dot Blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immobilized Recombinant SARS-COV-2 Nucleocapsid (RP01264) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Antibody (A18797) with a linear range of 3.12-200ng/mL.

ABclonal:Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Nucleoprotein antibody (A18797). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (A18797) at a dilution of 1:1000.

ABclonal:Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Nucleoprotein antibody (A18797). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (A18797) at a dilution of 1:3000.

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Overview

Product name SARS-CoV-2 Nucleoprotein Rabbit pAb
Catalog No. A18797
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of coronavirus Nucleoprotein (P0DTC9).
Sequence MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Gene ID 43740575
Swiss prot
Synonyms
Calculated MW
Observed MW 50kDa

Applications

Reactivity SARS-CoV-2
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • DB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples 293T-N, SARS-CoV Nucleoprotein NP protein(His-Tag)
Cellular location
Customer validation

IF (Homo sapiens)

Co-IP (Homo sapiens)

WB (Homo sapiens)

IB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

SARS-CoV-2 Nucleoprotein Rabbit pAb images

ABclonal:Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)}

Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)}

Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2,using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Dot Blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)}

Dot Blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immobilized Recombinant SARS-COV-2 Nucleocapsid (RP01264) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Antibody (A18797) with a linear range of 3.12-200ng/mL.
ABclonal:Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)}

Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)}

Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Nucleoprotein antibody (A18797). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (A18797) at a dilution of 1:1000.
ABclonal:Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)}

Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Nucleoprotein antibody (A18797). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (A18797) at a dilution of 1:3000.

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Related Targets

Based on existing publications, the following genes are closely related to research on Nucleoprotein. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Nucleoprotein. (Distance between topics and target gene indicate popularity.) Nucleoprotein

* Data provided by citexs.com, for reference only.

Publishing research using A18797? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Proteins (5)

ELISA Kits (1)

Secondary Antibodies (23)

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