Product Type > Proteins

Recombinant Mouse B7-H1/PD-L1/CD274 Protein (RP01149)

Datasheet

Overview

Product name Recombinant Mouse B7-H1/PD-L1/CD274 Protein
Catalog No. RP01149
Description Recombinant Mouse B7-H1/PD-L1/CD274 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe19-Thr238) of mouse PD-L1/B7-H1 (Accession #NP_068693) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-1 at 10 μg/mL (100 μL/well) can bind Recombinant Mouse PD-L1 with a linear range of 1.5-5 μg/mL.
Purity > 97% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Mouse
Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1). PD-L1/B7-H1 is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma.
Synonym PD-; B7h1; Pdl1; Pdcd1l; Pdcd1l1; Pdcd1lg1; A530045L16Rik; CD274
Expressed Host HEK293 cells
Tag C-hFc&His
Swiss-Prot Q9EP73
Gene ID 60533
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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