Product Type > Proteins

Recombinant Human TNFSF13B/BAFF/CD257 Protein (RP00018)

Datasheet

Overview

Product name Recombinant Human TNFSF13B/BAFF/CD257 Protein
Catalog No. RP00018
Description Recombinant Human TNFSF13B/BAFF/CD257 Protein is produced by E. coli expression system. The target protein is expressed with sequence ( Ala134-Leu285) of human TNFSF13B (Accession #NP_006564.1).
Bio-Activity 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 5 μg/mL (100 μL/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL.
2.Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1-1 ng/mL, corresponding to a specific activity of 1×106-1×107units/mg.
3.Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His (Catalog: RP00155) on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag (Catalog: RP00018) with an affinity constant of 3.05nM as determined in BLI assay (Gator).
Purity > 90% by SDS-PAGE.
Endotoxin Please contact us for more information.
Formulation Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation.
Species Human
The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.
Synonym TNFSF13B; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; TNLG7A; ZTNF4
Expressed Host E. coli
Tag No tag
Swiss-Prot Q9Y275
Gene ID 10673
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Inquire About This Product

Submit your question about RP00018 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order