Product Type > Proteins

Recombinant Human IL-1 alpha Protein (RP00098)

Datasheet COA

Overview

Product name Recombinant Human IL-1 alpha Protein
Catalog No. RP00098
Description Recombinant Human IL-1 alpha Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser113-Ala271) of human IL-1 alpha/IL1A/IL1F1 (Accession #NP_000566.3) fused with a 6×His tag at the C-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL1A at 5 μg/mL (100 μL/well) can bind recombinant Human IL1R2, the ED50 of Human IL1R2 is 2.18 μg/mL.
Purity > 95% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
Synonym IL1A; IL-1A; IL1; IL1-ALPHA; IL1F1
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot P01583
Gene ID 3552
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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