Product Type > Proteins

Recombinant Human BTN1A1/Butyrophilin Protein (RP00249)

Datasheet

Overview

Product name Recombinant Human BTN1A1/Butyrophilin Protein
Catalog No. RP00249
Description Recombinant Human BTN1A1/Butyrophilin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 27 - Arg 242 ) of human BTN1A1/Butyrophilin (Accession #NP_001723.2) fused with a 6×His tag at the C-terminus.
Bio-Activity Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat cells. The ED50 for this effect is 3.35-13.4 ng/mL.
Purity > 97% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species Human
Synonym BTN1A1; BT; BTN; BTN1
Expressed Host HEK293 cells
Tag C-His
Swiss-Prot Q13410
Gene ID 696
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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