Product name | Recombinant Human B7-H4/VTCN1 Protein |
---|---|
Catalog No. | RP00218 |
Description | Recombinant Human B7-H4/VTCN1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe29-Ala258) of human B7-H4/B7S1/B7x (Accession #NP_078902.2) fused with a 6×His tag at the C-terminus. |
Bio-Activity |
Measured by its binding ability in a functional ELISA.Immobilized Mouse B7-H4/VTCN1/VISTA (Catalog: RP00218) at 1 μg/mL (100 μL/well) can bind B7-H4/VTCN1 Rabbit pAb with a linear range of 0.2-22 ng/mL. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | Human |
Storage | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
---|---|
Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Submit your question about RP00218 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
* For research use only. Not for therapeutic or diagnostic purposes.