Product Type > Proteins

Recombinant SARS-CoV-2 Spike S1 Protein (RP01259)

Datasheet

ABclonal:Recombinant SARS-CoV-2 Spike S1 Protein

Overview

Product name Recombinant SARS-CoV-2 Spike S1 Protein
Catalog No. RP01259
Description Recombinant SARS-CoV-2(2019-nCoV) Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of SARS-COV-2(2019-nCoV) Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/mL.
2.Immobilized Human ACE2 on COOH Chip can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay (Nicoya OpenSPR).
Purity > 90% by SDS-PAGE.
Endotoxin < 0.1 EU/μg of the protein by LAL method.
Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Species SARS-CoV-2
Calculated MW 103kDa
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Synonym Envelope; SARS-CoV-2 Spike RBD (N501Y); Spike; Spike ECD; Spike RBD; Spike S1; Spike S2; Spike S2 ECD; S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein
Expressed Host HEK293 cells
Tag C-hFc&His
Gene ID 43740568
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
or This product is stable at ≤ -70°C for up to 6 months from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Reconstitution Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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Proteins (19)

Antibodies (12)

ELISA Kits (1)

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