Catalog No:AE011
Host species:Rabbit
Purity:Affinity purification
ABclonal:Western blot - GFP-Tag antibody (AE011)

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Immunogen information

ImmunogenRecombinant protein of GFP
SequenceEmail for sequence
Gene ID
Swiss Prot
Calculated MW
Observed MW


Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:2000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular Location
Customer Validation

WB (Arabidopsis, Human)

IF (Human)

IP (Human)

Rabbit anti GFP-Tag pAb images

ABclonal:Western blot - GFP-Tag antibody (AE011)

Western blot - GFP-Tag antibody (AE011)

Western blot analysis of over-expressed GFP fusion protein in 293 cell using GFP-Tag antibody (AE011) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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