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Rabbit anti GFP-Tag pAb (AE011)

Publications (5) Datasheet

ABclonal:Western blot - Rabbit anti GFP-Tag pAb (AE011)


Product nameRabbit anti GFP-Tag pAb
Catalog No.AE011
Host speciesRabbit
Purification methodAffinity purification
ImmunogenRecombinant protein of GFP
SequenceEmail for sequence
Gene ID
Swiss prot
Calculated MW
Observed MW
Recommended dilutionWB 1:2000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular location
Customer validation

WB(Human, Arabidopsis)




ABclonal:Western blot - Rabbit anti GFP-Tag pAb (AE011) }

Western blot - Rabbit anti GFP-Tag pAb (AE011)

Western blot analysis of extracts of normal 293T cells and 293T transfected with EGFP, using GFP antibody (AE011) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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