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Rabbit anti GFP-Tag pAb (AE011)

Publications (5) Datasheet

ABclonal:Western blot - Rabbit anti GFP-Tag pAb (AE011)

Overview

Product nameRabbit anti GFP-Tag pAb
Catalog No.AE011
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant protein of GFP
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:2000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular location
Customer validation

WB(Human, Arabidopsis)

IF(Human)

IP(Human)

IF(Human)

ABclonal:Western blot - Rabbit anti GFP-Tag pAb (AE011) }

Western blot - Rabbit anti GFP-Tag pAb (AE011)

Western blot analysis of extracts of normal 293T cells and 293T transfected with EGFP, using GFP antibody (AE011) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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