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RPS6 Polyclonal Antibody (A6058)

Publication (1) Datasheet

ABclonal:Western blot - RPS6 Polyclonal Antibody (A6058)

Overview

Product nameRPS6 Polyclonal Antibody
Catalog No.A6058
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS6 (NP_001001.2).
SequenceMKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGC
Gene ID6194
Swiss protP62753
SynonymsRPS6; S6; ribosomal protein S6
Calculated MW28kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHT-29, A-431, HeLa, NIH/3T3, Mouse liver, Rat liver
Cellular location
Customer validation

WB(Mouse)

ABclonal:Western blot - RPS6 Polyclonal Antibody (A6058) }

Western blot - RPS6 Polyclonal Antibody (A6058)

Western blot analysis of extracts of various cell lines, using RPS6 antibody (A6058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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