Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

RPS14 Rabbit pAb (A6727)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS14 Rabbit pAb (A6727)

Western blot analysis of extracts of various cell lines, using RPS14 antibody (A6727) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RPS14 Rabbit pAb (A6727)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using RPS14 Rabbit pAb (A6727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPS14 Rabbit pAb (A6727)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using RPS14 Rabbit pAb (A6727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RPS14 Rabbit pAb (A6727)

Immunofluorescence analysis of MCF7 cells using RPS14 antibody (A6727). Blue: DAPI for nuclear staining.

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Overview

Product name RPS14 Rabbit pAb
Catalog No. A6727
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human RPS14 (NP_005608.1).
Sequence MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL
Gene ID 6208
Swiss prot P62263
Synonyms S14; EMTB; uS11; RPS14
Calculated MW 16kDa
Observed MW 16kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples MCF7, SW480, HeLa, HepG2, Jurkat, Mouse spleen, Mouse lung, Rat spleen, HL-60
Cellular location cytoplasm, cytosol, cytosolic ribosome, extracellular exosome, focal adhesion, nucleolus, nucleoplasm

Documents

Certificate of Compliance

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Lot number

RPS14 Rabbit pAb images

ABclonal:Western blot - RPS14 Rabbit pAb (A6727)}

Western blot - RPS14 Rabbit pAb (A6727)

Western blot analysis of extracts of various cell lines, using RPS14 antibody (A6727) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - RPS14 Rabbit pAb (A6727)}

Immunohistochemistry - RPS14 Rabbit pAb (A6727)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using RPS14 Rabbit pAb (A6727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPS14 Rabbit pAb (A6727)}

Immunohistochemistry - RPS14 Rabbit pAb (A6727)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using RPS14 Rabbit pAb (A6727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RPS14 Rabbit pAb (A6727)}

Immunofluorescence - RPS14 Rabbit pAb (A6727)

Immunofluorescence analysis of MCF7 cells using RPS14 antibody (A6727). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on RPS14. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RPS14. (Distance between topics and target gene indicate popularity.) RPS14

* Data provided by citexs.com, for reference only.

Publishing research using A6727? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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