Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

RPL36 Rabbit pAb (A7793)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - RPL36 Rabbit pAb (A7793)

Western blot analysis of extracts of various cell lines, using RPL36 antibody (A7793) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - RPL36 Rabbit pAb (A7793)

Immunohistochemistry analysis of paraffin-embedded rat brain using RPL36 Rabbit pAb (A7793) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL36 Rabbit pAb (A7793)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using RPL36 Rabbit pAb (A7793) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL36 Rabbit pAb (A7793)

Immunohistochemistry analysis of paraffin-embedded mouse heart using RPL36 Rabbit pAb (A7793) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RPL36 Rabbit pAb (A7793)

Immunofluorescence analysis of U2OS cells using RPL36 antibody (A7793) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL36 Rabbit pAb (A7793)

Confocal immunofluorescence analysis of HeLa cells using RPL36 antibody (A7793) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL36 Rabbit pAb (A7793)

Confocal immunofluorescence analysis of HeLa cells using RPL36 Polyclonal Antibody (A7793) at dilution of 1:200. Blue: DAPI for nuclear staining.

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Overview

Product name RPL36 Rabbit pAb
Catalog No. A7793
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L36E family of ribosomal proteins. It is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human RPL36 (NP_056229.2).
Sequence MALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFAPYERRAMELLKVSKDKRALKFIKKRVGTHIRAKRKREELSNVLAAMRKAAAKKD
Gene ID 25873
Swiss prot Q9Y3U8
Synonyms L36; eL36; RPL36
Calculated MW 12kDa
Observed MW 15kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples PC-12, HepG2, Jurkat, HeLa, Mouse spleen, Mouse lung, Rat spleen, Rat lung
Cellular location cytoplasm, cytosol, cytosolic ribosome, endoplasmic reticulum, nucleolus

Documents

Certificate of Compliance

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Lot number

Research Area

RPL36 Rabbit pAb images

ABclonal:Western blot - RPL36 Rabbit pAb (A7793)}

Western blot - RPL36 Rabbit pAb (A7793)

Western blot analysis of extracts of various cell lines, using RPL36 antibody (A7793) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - RPL36 Rabbit pAb (A7793)}

Immunohistochemistry - RPL36 Rabbit pAb (A7793)

Immunohistochemistry analysis of paraffin-embedded rat brain using RPL36 Rabbit pAb (A7793) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL36 Rabbit pAb (A7793)}

Immunohistochemistry - RPL36 Rabbit pAb (A7793)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using RPL36 Rabbit pAb (A7793) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL36 Rabbit pAb (A7793)}

Immunohistochemistry - RPL36 Rabbit pAb (A7793)

Immunohistochemistry analysis of paraffin-embedded mouse heart using RPL36 Rabbit pAb (A7793) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RPL36 Rabbit pAb (A7793)}

Immunofluorescence - RPL36 Rabbit pAb (A7793)

Immunofluorescence analysis of U2OS cells using RPL36 antibody (A7793) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPL36 Rabbit pAb (A7793)}

Immunofluorescence - RPL36 Rabbit pAb (A7793)

Confocal immunofluorescence analysis of HeLa cells using RPL36 antibody (A7793) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPL36 Rabbit pAb (A7793)}

Immunofluorescence - RPL36 Rabbit pAb (A7793)

Confocal immunofluorescence analysis of HeLa cells using RPL36 Polyclonal Antibody (A7793) at dilution of 1:200. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on RPL36. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to RPL36. (Distance between topics and target gene indicate popularity.) RPL36

* Data provided by citexs.com, for reference only.

Publishing research using A7793? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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