Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

RHBDF1 Rabbit pAb (A17754)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - RHBDF1 Rabbit pAb (A17754)

Western blot analysis of various lysates using RHBDF1 Rabbit pAb (A17754) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - RHBDF1 Rabbit pAb (A17754)

Immunofluorescence analysis of L929 cells using RHBDF1 Rabbit pAb (A17754) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RHBDF1 Rabbit pAb (A17754)

Immunofluorescence analysis of L929 cells using RHBDF1 Rabbit pAb (A17754) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name RHBDF1 Rabbit pAb
Catalog No. A17754
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable growth factor binding activity and serine-type endopeptidase activity. Involved in several processes, including negative regulation of protein secretion; regulation of epidermal growth factor receptor signaling pathway; and regulation of proteasomal protein catabolic process. Located in Golgi membrane and endoplasmic reticulum membrane.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human RHBDF1 (NP_071895.3).
Sequence HRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMD
Gene ID 64285
Swiss prot Q96CC6
Synonyms Dist1; hDist1; C16orf8; EGFR-RS; gene-89; gene-90; RHBDF1
Calculated MW 97kDa
Observed MW 97kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse lung, Mouse heart, Mouse kidney, Rat lung, Rat heart, Rat kidney
Cellular location

Documents

Certificate of Compliance

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Lot number

RHBDF1 Rabbit pAb images

ABclonal:Western blot - RHBDF1 Rabbit pAb (A17754)}

Western blot - RHBDF1 Rabbit pAb (A17754)

Western blot analysis of various lysates using RHBDF1 Rabbit pAb (A17754) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - RHBDF1 Rabbit pAb (A17754)}

Immunofluorescence - RHBDF1 Rabbit pAb (A17754)

Immunofluorescence analysis of L929 cells using RHBDF1 Rabbit pAb (A17754) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RHBDF1 Rabbit pAb (A17754)}

Immunofluorescence - RHBDF1 Rabbit pAb (A17754)

Immunofluorescence analysis of L929 cells using RHBDF1 Rabbit pAb (A17754) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on RHBDF1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RHBDF1. (Distance between topics and target gene indicate popularity.) RHBDF1

* Data provided by citexs.com, for reference only.

Publishing research using A17754? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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