Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Reviews (2) Publications (18) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Western blot analysis of C6, using Phospho-c-Jun-S63 Rabbit pAb (AP0048) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for Phospho-c-Jun-S63 Rabbit pAb(AP0048)
Review
ABclonal: review for Phospho-c-Jun-S63 Rabbit pAb(AP0048)

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Overview

Product name Phospho-c-Jun-S63 Rabbit pAb
Catalog No. AP0048
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S63 of human c-Jun (NP_002219.1).
Sequence LTSPD
Gene ID 3725
Swiss prot P05412
Synonyms AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S63
Calculated MW 36kDa
Observed MW 48kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples C6+UV
Cellular location Nucleus
Customer validation

WB (Mouse embryonal fibroblast cell, 293THomo sapiens, Homo sapiens, Mus musculus, Sus scrofa, Chlorocebus aethiops, Gallus gallus, Rattus norvegicus, Oryza sativa)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-c-Jun-S63 Rabbit pAb images

ABclonal:Western blot - Phospho-c-Jun-S63 Rabbit pAb (AP0048)}

Western blot - Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Western blot analysis of C6, using Phospho-c-Jun-S63 Rabbit pAb (AP0048) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on JUN. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to JUN. (Distance between topics and target gene indicate popularity.) JUN

* Data provided by citexs.com, for reference only.

Publishing research using AP0048? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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