Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Publications (7) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of various cell lines, using Phospho-beta Catenin-S552 antibody (AP0579) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of C2C12 cells, using Phospho-β-Catenin-S552 pAb (AP0579) at 1:1000 dilution or β-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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Overview

Product name Phospho-β-Catenin-S552 Rabbit pAb
Catalog No. AP0579
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S552 of human beta Catenin (NP_001091679).
Sequence RTSMG
Gene ID 1499
Swiss prot P35222
Synonyms EVR7; CTNNB; MRD19; NEDSDV; armadillo; Phospho-β-Catenin-S552
Calculated MW 85kDa
Observed MW 106kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples C2C12, NIH/3T3
Cellular location Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

WB (Homo sapiens, Rattus norvegicus)

IHC (Mus musculus)

IF (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-β-Catenin-S552 Rabbit pAb images

ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)}

Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of various cell lines, using Phospho-beta Catenin-S552 antibody (AP0579) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)}

Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Western blot analysis of extracts of C2C12 cells, using Phospho-β-Catenin-S552 pAb (AP0579) at 1:1000 dilution or β-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CTNNB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CTNNB1. (Distance between topics and target gene indicate popularity.) CTNNB1

* Data provided by citexs.com, for reference only.

Publishing research using AP0579? Please let us know so that we can cite the reference in this datasheet.

Antibodies (15)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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