Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Publications (16) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Western blot analysis of A-431, using Phospho-VEGF Receptor 2-Y1175 antibody (AP0382) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name Phospho-VEGF Receptor 2-Y1175 Rabbit pAb
Catalog No. AP0382
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y1175 of human VEGF Receptor 2 (NP_002244.1).
Sequence KDYIV
Gene ID 3791
Swiss prot P35968
Synonyms FLK1; CD309; VEGFR; VEGFR2; Phospho-VEGF Receptor 2-Y1175
Calculated MW 152kDa
Observed MW 230kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A-431
Cellular location Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Early endosome, Endoplasmic reticulum, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation

WB (Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus)

IF (Gallus gallus, Homo sapiens, Rattus norvegicus)

IHC (Homo sapiens)

RT-PCR (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb images

ABclonal:Western blot - Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)}

Western blot - Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Western blot analysis of A-431, using Phospho-VEGF Receptor 2-Y1175 antibody (AP0382) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on KDR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KDR. (Distance between topics and target gene indicate popularity.) KDR

* Data provided by citexs.com, for reference only.

Publishing research using AP0382? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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