Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)

Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 antibody (AP0220) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)

Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 antibody (AP0220) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)

Immunohistochemistry analysis of Stathmin 1 in paraffin-embedded human breast carcinoma using Phospho-Stathmin 1-S25 antibody (AP0220).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-Stathmin 1-S25 Rabbit pAb
Catalog No. AP0220
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S25 of human Stathmin 1 (NP_005554.1).
Sequence ILSPRS
Gene ID 3925
Swiss prot P16949
Synonyms Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215; Phospho-Stathmin 1-S25
Calculated MW 17kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Jurkat, C6
Cellular location Cytoplasm, cytoskeleton
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-Stathmin 1-S25 Rabbit pAb images

ABclonal:Western blot - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)}

Western blot - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)

Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 antibody (AP0220) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)}

Western blot - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)

Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 antibody (AP0220) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)}

Immunohistochemistry - Phospho-Stathmin 1-S25 Rabbit pAb (AP0220)

Immunohistochemistry analysis of Stathmin 1 in paraffin-embedded human breast carcinoma using Phospho-Stathmin 1-S25 antibody (AP0220).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on STMN1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to STMN1. (Distance between topics and target gene indicate popularity.) STMN1

* Data provided by citexs.com, for reference only.

Publishing research using AP0220? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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