Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)

Reviews (2) Publications (13) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)

Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)

Immunohistochemistry analysis of Phospho-p38 MAPK-Y182 in paraffin-embedded human colon carcinoma tissue using Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)

Immunohistochemistry analysis of Phospho-p38 MAPK-Y182 in paraffin-embedded rat lung tissue using Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Review
ABclonal: review for Phospho-p38 MAPK-Y182 Rabbit pAb(AP0057)
Review
ABclonal: review for Phospho-p38 MAPK-Y182 Rabbit pAb(AP0057)

You may also interested in:

Overview

Product name Phospho-p38 MAPK-Y182 Rabbit pAb
Catalog No. AP0057
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y182 of human MAPK (NP_620581.1).
Sequence MTGYVA
Gene ID 1432
Swiss prot Q16539
Synonyms RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; Phospho-p38 MAPK-Y182
Calculated MW 41kDa
Observed MW 42kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T
Cellular location Cytoplasm, Nucleus
Customer validation

IHC (A549,Mouse kidney)

WB (A549,Mouse kidneyHomo sapiens, Homo sapiens, Rattus norvegicus, Bos taurus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-p38 MAPK-Y182 Rabbit pAb images

ABclonal:Western blot - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)}

Western blot - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)

Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)}

Immunohistochemistry - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)

Immunohistochemistry analysis of Phospho-p38 MAPK-Y182 in paraffin-embedded human colon carcinoma tissue using Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)}

Immunohistochemistry - Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057)

Immunohistochemistry analysis of Phospho-p38 MAPK-Y182 in paraffin-embedded rat lung tissue using Phospho-p38 MAPK-Y182 Rabbit pAb (AP0057) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Submit an review for AP0057

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about AP0057 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on MAPK14. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK14. (Distance between topics and target gene indicate popularity.) MAPK14

* Data provided by citexs.com, for reference only.

Publishing research using AP0057? Please let us know so that we can cite the reference in this datasheet.

Antibodies (12)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order