Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)

Western blot analysis of various lysates using Phospho-Lamin A/C-S22 Rabbit pAb (AP0777) at 1:2000 dilution or Lamin A antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Lamin A/C-S22 pAb (AP0777). Western blot was performed from the immunoprecipitate using Phospho-Lamin A/C-S22 pAb (AP0777) at a dilution of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.

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Overview

Product name Phospho-Lamin A/C-S22 Rabbit pAb
Catalog No. AP0777
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S22 of human Lamin A (NP_005563.1).
Sequence PLSPT
Gene ID 4000
Swiss prot P02545
Synonyms FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; Phospho-Lamin A/C-S22
Calculated MW 74kDa
Observed MW 69kDa, 78kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples HeLa, C6
Cellular location Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-Lamin A/C-S22 Rabbit pAb images

ABclonal:Western blot - Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)}

Western blot - Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)

Western blot analysis of various lysates using Phospho-Lamin A/C-S22 Rabbit pAb (AP0777) at 1:2000 dilution or Lamin A antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.
ABclonal:Immunoprecipitation - Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)}

Immunoprecipitation - Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Lamin A/C-S22 pAb (AP0777). Western blot was performed from the immunoprecipitate using Phospho-Lamin A/C-S22 pAb (AP0777) at a dilution of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.

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Related Targets

Based on existing publications, the following genes are closely related to research on LMNA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to LMNA. (Distance between topics and target gene indicate popularity.) LMNA

* Data provided by citexs.com, for reference only.

Publishing research using AP0777? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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