Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Cyclin D1-T286 Rabbit pAb (AP0574)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Phospho-Cyclin D1-T286 Rabbit pAb (AP0574)

Western blot analysis of extracts of various cell lines, using Phospho-Cyclin D1-T286 pAb (AP0574) at 1:1000 dilution.C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

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Overview

Product name Phospho-Cyclin D1-T286 Rabbit pAb
Catalog No. AP0574
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T286 of human Cyclin D1 (NP_444284.1).
Sequence ACTPT
Gene ID 595
Swiss prot P24385
Synonyms BCL1; PRAD1; U21B31; D11S287E; Phospho-Cyclin D1-T286
Calculated MW 34kDa
Observed MW 36kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples C6
Cellular location Cytoplasm, Membrane, Nucleus

Research Area

Phospho-Cyclin D1-T286 Rabbit pAb images

ABclonal:Western blot - Phospho-Cyclin D1-T286 Rabbit pAb (AP0574)}

Western blot - Phospho-Cyclin D1-T286 Rabbit pAb (AP0574)

Western blot analysis of extracts of various cell lines, using Phospho-Cyclin D1-T286 pAb (AP0574) at 1:1000 dilution.C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCND1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCND1. (Distance between topics and target gene indicate popularity.) CCND1

* Data provided by citexs.com, for reference only.

Publishing research using AP0574? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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