Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 Rabbit pAb (AP0274)

Publications (3) Datasheet

ABclonal:Western blot - Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 Rabbit pAb (AP0274)

Western blot analysis of extracts HepG2 cells using Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 antibody (AP0274).HepG2 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 Rabbit pAb
Catalog No.AP0274
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
ImmunogenA phospho specific peptide corresponding to residues surrounding Y315 of human AKT1
SequenceEmail for sequence
Gene ID207/208/10000
Swiss protP31749/P31751/Q9Y243
SynonymsAKT1/AKT2/AKT3
Calculated MW48kDa/55kDa/51kDa/54kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular location
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Rattus norvegicus, Homo sapiens)

    ABclonal:Western blot - Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 Rabbit pAb (AP0274) }

    Western blot - Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 Rabbit pAb (AP0274)

    Western blot analysis of extracts HepG2 cells using Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 antibody (AP0274).HepG2 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Inquire About This Product

    Submit your question about AP0274 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Publishing research using AP0274? Please let us know so that we can cite the reference in this datasheet.

    Select size and quantity
    Contact us to order