Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-p53-S15 Rabbit pAb (AP0083)

Reviews (2) Publications (15) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunofluorescence - Phospho-p53-S15 Rabbit pAb (AP0083)

Immunofluorescence analysis of HT-29 treated with UV and HT-29 cells using Phospho-p53-S15 Rabbit pAb (AP0083) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-p53-S15 Rabbit pAb(AP0083)
Review
ABclonal: review for Phospho-p53-S15 Rabbit pAb(AP0083)

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Overview

Product name Phospho-p53-S15 Rabbit pAb
Catalog No. AP0083
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen A synthetic phosphorylated peptide around S15 of human p53 (NP_000537.3).
Sequence PLSQE
Gene ID 7157
Swiss prot P04637
Synonyms P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-S15
Calculated MW 44kDa
Observed MW 53kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T+UV
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

WB (Adenocarcinoma human alveolar basal epithelial cells, A549Homo sapiens, Homo sapiens, Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-p53-S15 Rabbit pAb images

ABclonal:Immunofluorescence - Phospho-p53-S15 Rabbit pAb (AP0083)}

Immunofluorescence - Phospho-p53-S15 Rabbit pAb (AP0083)

Immunofluorescence analysis of HT-29 treated with UV and HT-29 cells using Phospho-p53-S15 Rabbit pAb (AP0083) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53

* Data provided by citexs.com, for reference only.

Publishing research using AP0083? Please let us know so that we can cite the reference in this datasheet.

Antibodies (31)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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