Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Publications (43) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Western blot analysis of lysates from C2C12 cells, using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at 1:800 dilution.C2C12 cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded human colon carcinoma using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded human esophageal cancer using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded mouse stomach using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded rat spleen using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of L929 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of PC-12 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of U2OS cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Overview

Product name Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb
Catalog No. AP0548
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Immunogen information

Immunogen A synthetic phosphorylated peptide around S465 & S467 of human Phospho-Smad2-S465/467 + Smad3-S423/425SMAD2 (NP_005892.1).
Sequence RCSSMS
Gene ID 40874088
Swiss prot Q15796P84022
Synonyms HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; Phospho-Smad2-S465/467 + Smad3-S423/425
Calculated MW 48kDa/52kDa/25kDa/35kDa/43kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples C2C12
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus, Homo sapiensRattus norvegicus, Gallus gallus)

IF (Homo sapiens, Gallus gallus, Rattus norvegicus)

IHC (Mus musculus, Rattus norvegicus)

ChIP (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb images

ABclonal:Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Western blot analysis of lysates from C2C12 cells, using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at 1:800 dilution.C2C12 cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded human colon carcinoma using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded human esophageal cancer using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded mouse stomach using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of Phospho-Smad2-S465/467 + Smad3-S423/425 in paraffin-embedded rat spleen using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of L929 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of PC-12 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of U2OS cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on SMAD2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SMAD2. (Distance between topics and target gene indicate popularity.) SMAD2

* Data provided by citexs.com, for reference only.

Publishing research using AP0548? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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