Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-RB-S811 Rabbit pAb (AP0089)

Publications (3) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-RB-S811 Rabbit pAb (AP0089)

Western blot analysis of extracts of HeLa cells, using Phospho-RB-S811 antibody (AP0089) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-RB-S811 Rabbit pAb
Catalog No. AP0089
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S811 of human RB (NP_000312.2).
Sequence LKSPY
Gene ID 5925
Swiss prot P06400
Synonyms RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1; Phospho-RB-S811
Calculated MW 106kDa
Observed MW 106kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Nucleus
Customer validation

WB (Mus musculus, Homo sapiens)

Research Area

Phospho-RB-S811 Rabbit pAb images

ABclonal:Western blot - Phospho-RB-S811 Rabbit pAb (AP0089)}

Western blot - Phospho-RB-S811 Rabbit pAb (AP0089)

Western blot analysis of extracts of HeLa cells, using Phospho-RB-S811 antibody (AP0089) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on RB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RB1. (Distance between topics and target gene indicate popularity.) RB1

* Data provided by citexs.com, for reference only.

Publishing research using AP0089? Please let us know so that we can cite the reference in this datasheet.

Antibodies (13)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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