Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Review (1) Publications (32) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. HeLa cells were treated by TNF-α (50 ng/ml) and Calyculin A (50 nM) at 37℃ for 10 minutes.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 30s.

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. NIH/3T3 cells were treated by TNF-α (10 ng/ml) at 37℃ for 30 minutes.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. C6 cells were treated by TNF-α (50 ng/ml) at 37℃ for 30 minutes.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.

ABclonal:Immunofluorescence - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Immunofluorescence analysis of HeLa TNFα + CA and HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-NF-kB p65/RelA-S536 Rabbit pAb(AP0124)

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Overview

Product name Phospho-NF-kB p65/RelA-S536 Rabbit pAb
Catalog No. AP0124
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S536 of human RelA (NP_068810.3).
Sequence FSSIA
Gene ID 5970
Swiss prot Q04206
Synonyms p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S536
Calculated MW 60kDa
Observed MW 65kDa/

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa+CA+TNF-α, NIH/3T3+TNF-α, C6+TNF-α, C6, HeLa, NIH/3T3
Cellular location Cytoplasm, Nucleus
Customer validation

WB (CAL27Homo sapiens, Homo sapiens, Mus musculus, Sus scrofa, Plant, Rattus norvegicus, King Charles Spaniel)

IHC (Mus musculus, Rattus norvegicus)

ELISA (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-NF-kB p65/RelA-S536 Rabbit pAb images

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)}

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. HeLa cells were treated by TNF-α (50 ng/ml) and Calyculin A (50 nM) at 37℃ for 10 minutes.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 30s.
ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)}

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. NIH/3T3 cells were treated by TNF-α (10 ng/ml) at 37℃ for 30 minutes.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.
ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)}

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. C6 cells were treated by TNF-α (50 ng/ml) at 37℃ for 30 minutes.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.
ABclonal:Immunofluorescence - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)}

Immunofluorescence - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Immunofluorescence analysis of HeLa TNFα + CA and HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on RELA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RELA. (Distance between topics and target gene indicate popularity.) RELA

* Data provided by citexs.com, for reference only.

Publishing research using AP0124? Please let us know so that we can cite the reference in this datasheet.

Antibodies (22)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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