Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Reviews (4) Publications (30) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunohistochemistry analysis of Phospho-AMPKα1-T183/AMPKα2-T172 in paraffin-embedded rat liver using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunohistochemistry analysis of Phospho-AMPKα1-T183/AMPKα2-T172 in paraffin-embedded human lung cancer using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunohistochemistry analysis of Phospho-AMPKα1-T183/AMPKα2-T172 in paraffin-embedded mouse brain using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunoprecipitation analysis of 200 μg extracts of C2C12 cells, using 3 μg Phospho-AMPKα1-T183/AMPKα2-T172 pAb (AP0116). Western blot was performed from the immunoprecipitate using Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116) at a dilution of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.

Review
ABclonal: review for Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb(AP0116)
Review
ABclonal: review for Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb(AP0116)
Review
ABclonal: review for Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb(AP0116)
Review
ABclonal: review for Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb(AP0116)

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Overview

Product name Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb
Catalog No. AP0116
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T172 of human AMPKα
Sequence Email for sequence
Gene ID 55625563
Swiss prot Q13131P54646
Synonyms AMPKa1/AMPKa2; Phospho-AMPKα1-T183/AMPKα2-T172
Calculated MW 64kDa/65kDa
Observed MW 64kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples C2C12
Cellular location apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus
Customer validation

WB (Human lung cancer cells, unknow, HeLaHomo sapiens, Rattus norvegicus, Mus musculus, Danio rerio, Homo sapiens, Cavia porcellus, Capra hircus)

IHC (Human lung cancer cellsHomo sapiens, Capra hircus)

IF (Rattus norvegicus)

mIHC/mICC (Capra hircus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb images

ABclonal:Western blot - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)}

Western blot - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)}

Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunohistochemistry analysis of Phospho-AMPKα1-T183/AMPKα2-T172 in paraffin-embedded rat liver using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)}

Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunohistochemistry analysis of Phospho-AMPKα1-T183/AMPKα2-T172 in paraffin-embedded human lung cancer using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)}

Immunohistochemistry - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunohistochemistry analysis of Phospho-AMPKα1-T183/AMPKα2-T172 in paraffin-embedded mouse brain using Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)}

Immunoprecipitation - Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb (AP0116)

Immunoprecipitation analysis of 200 μg extracts of C2C12 cells, using 3 μg Phospho-AMPKα1-T183/AMPKα2-T172 pAb (AP0116). Western blot was performed from the immunoprecipitate using Phospho-AMPKa1-T183/AMPKa2-T172 pAb (AP0116) at a dilution of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKAA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKAA1. (Distance between topics and target gene indicate popularity.) PRKAA1

* Data provided by citexs.com, for reference only.

Publishing research using AP0116? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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