Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)

Reviews (2) Publications (21) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)

Western blot analysis of various lysates, using Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473) at 1:5000 dilution.293T, NIH/3T3 and C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Review
ABclonal: review for Phospho-JNK1/2-T183/Y185 Rabbit pAb(AP0473)
Review
ABclonal: review for Phospho-JNK1/2-T183/Y185 Rabbit pAb(AP0473)

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Overview

Product name Phospho-JNK1/2-T183/Y185 Rabbit pAb
Catalog No. AP0473
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Immunogen information

Immunogen A synthetic phosphorylated peptide around T183 & Y185 of human JNK1 (P45983).
Sequence MTPYV
Gene ID 55995601
Swiss prot P45983P45984
Synonyms JNK1/2; Phospho-JNK1/2-T183/Y185
Calculated MW 35kDa/44kDa/48kDa/27kDa
Observed MW 46kDa/54kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T+UV, NIH/3T3+UV, C6+UV
Cellular location axon, basal dendrite, cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus, synapse
Customer validation

WB (Mouse embryonal fibroblast cell, Mouse heart tissueMus musculus, Mus musculus, Rattus norvegicus, Homo sapiens, Plant, Sus domesticus, Crayfish)

IHC (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-JNK1/2-T183/Y185 Rabbit pAb images

ABclonal:Western blot - Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)}

Western blot - Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)

Western blot analysis of various lysates, using Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473) at 1:5000 dilution.293T, NIH/3T3 and C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPK8. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK8. (Distance between topics and target gene indicate popularity.) MAPK8

* Data provided by citexs.com, for reference only.

Publishing research using AP0473? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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