Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-H2AFX-S139 pAb (AP0099)

Reviews (3) Datasheet

ABclonal:Western blot - Phospho-H2A.X-S139 antibody (AP0099)
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

Overview

Product namePhospho-H2AFX-S139 pAb
Catalog No.AP0099
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
ImmunogenA phospho specific peptide corresponding to residues surrounding S139 of human Histone H2A.x
SequenceEmail for sequence
Gene ID3014
Swiss protP16104
Synonyms
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, NIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

WB(U251 cells, N2A)

IF(Mouse bone marrow)

ABclonal:Western blot - Phospho-H2A.X-S139 antibody (AP0099)}

Western blot - Phospho-H2A.X-S139 antibody (AP0099)

Western blot analysis of extracts of various cell lines, using Phospho-H2A.X-S139 antibody (AP0099) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) for 5 hours. NIH/3T3 cells were treated by UV for 15-30 minutes. C6 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)}

Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

Immunofluorescence analysis of U2OS cells using Phospho-H2AFX-S139 antibody (AP0099). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)}

Immunofluorescence - Phospho-H2AFX-S139 antibody (AP0099)

Immunofluorescence analysis of U2OS cells using Phospho-H2AFX-S139 antibody (AP0099). Blue: DAPI for nuclear staining.

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