Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Publications (12) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Western blot analysis of various lysates using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at 1:1000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Western blot - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Western blot analysis of lysates from C6 cells, using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at 1:1000 dilution.C6 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Immunohistochemistry analysis of Phospho-eIF4EBP1-T37/46 in paraffin-embedded rat kidney using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Immunohistochemistry analysis of Phospho-eIF4EBP1-T37/46 in paraffin-embedded rat pancreas using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Immunofluorescence analysis of U-2 OS cells using Phospho-eIF4EBP1-T37/46 Rabbit pAb(AP0030) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name Phospho-eIF4EBP1-T37/46 Rabbit pAb
Catalog No. AP0030
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T37 & T46 of human eIF4EBP1 (NP_004086.1).
Sequence STTPGGTLFSTTPG
Gene ID 1978
Swiss prot Q13541
Synonyms BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T37/46
Calculated MW 13kDa
Observed MW 15kDa-20kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, NIH/3T3, C6
Cellular location cytoplasm, cytosol
Customer validation

WB (Macaca mulatta, Homo sapiens, Mus musculus, Bombyx mori Linnaeus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-eIF4EBP1-T37/46 Rabbit pAb images

ABclonal:Western blot - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)}

Western blot - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Western blot analysis of various lysates using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at 1:1000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Western blot - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)}

Western blot - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Western blot analysis of lysates from C6 cells, using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at 1:1000 dilution.C6 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)}

Immunohistochemistry - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Immunohistochemistry analysis of Phospho-eIF4EBP1-T37/46 in paraffin-embedded rat kidney using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)}

Immunohistochemistry - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Immunohistochemistry analysis of Phospho-eIF4EBP1-T37/46 in paraffin-embedded rat pancreas using Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)}

Immunofluorescence - Phospho-eIF4EBP1-T37/46 Rabbit pAb (AP0030)

Immunofluorescence analysis of U-2 OS cells using Phospho-eIF4EBP1-T37/46 Rabbit pAb(AP0030) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on EIF4EBP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EIF4EBP1. (Distance between topics and target gene indicate popularity.) EIF4EBP1

* Data provided by citexs.com, for reference only.

Publishing research using AP0030? Please let us know so that we can cite the reference in this datasheet.

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