Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Chk2-T68 Rabbit pAb (AP0590)

Reviews (3) Publications (8) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-Chk2-T68 Rabbit pAb (AP0590)

Western blot analysis of extracts of HeLa cells, using Phospho-Chk2-T68 antibody (AP0590). HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Review
ABclonal: review for Phospho-Chk2-T68 Rabbit pAb(AP0590)
Review
ABclonal: review for Phospho-Chk2-T68 Rabbit pAb(AP0590)
Review
ABclonal: review for Phospho-Chk2-T68 Rabbit pAb(AP0590)

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Overview

Product name Phospho-Chk2-T68 Rabbit pAb
Catalog No. AP0590
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T68 of human Chk2 (NP_009125.1).
Sequence VSTQE
Gene ID 11200
Swiss prot O96017
Synonyms CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; Phospho-Chk2-T68
Calculated MW 61kDa
Observed MW 62kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Nucleus, Nucleus, PML body, nucleoplasm
Customer validation

WB (Adenocarcinoma human alveolar basal epithelial cells, HCC827Homo sapiens, Homo sapiens, Sus scrofa)

IHC (Adenocarcinoma human alveolar basal epithelial cells)

Research Area

Phospho-Chk2-T68 Rabbit pAb images

ABclonal:Western blot - Phospho-Chk2-T68 Rabbit pAb (AP0590)}

Western blot - Phospho-Chk2-T68 Rabbit pAb (AP0590)

Western blot analysis of extracts of HeLa cells, using Phospho-Chk2-T68 antibody (AP0590). HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on CHEK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CHEK2. (Distance between topics and target gene indicate popularity.) CHEK2

* Data provided by citexs.com, for reference only.

Publishing research using AP0590? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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