Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Bad-S155 Rabbit pAb (AP0009)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

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Overview

Product name Phospho-Bad-S155 Rabbit pAb
Catalog No. AP0009
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S155 of mouse Bad (NP_031548.1).
Sequence RGSSA
Gene ID 572
Swiss prot Q92934
Synonyms BBC2; BCL2L8; Phospho-Bad-S155
Calculated MW 18kDa
Observed MW Refer to figures

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples
Cellular location Cytoplasm, Mitochondrion outer membrane
Customer validation

WB (Homo sapiens)

Research Area

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Related Targets

Based on existing publications, the following genes are closely related to research on BAD. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BAD. (Distance between topics and target gene indicate popularity.) BAD

* Data provided by citexs.com, for reference only.

Publishing research using AP0009? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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