Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Pan-Akt Rabbit pAb (A3145)

Review (1) Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Pan-Akt Rabbit pAb (A3145)

Western blot analysis of various lysates using Pan-Akt Rabbit pAb (A3145) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Review
ABclonal: review for Pan-Akt Rabbit pAb(A3145)

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Overview

Product name Pan-Akt Rabbit pAb
Catalog No. A3145
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Immunogen information

Immunogen A synthetic peptide of human AKT1/AKT2/AKT3
Sequence Email for sequence
Gene ID 20720810000
Swiss prot P31749P31751Q9Y243
Synonyms AKT1/AKT2/AKT3; Pan-Akt
Calculated MW 48kDa/55kDa/51kDa/54kDa
Observed MW 68kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A375, BT-474, Mouse thymus
Cellular location ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle
Customer validation

WB (Mouse GVMus musculus, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Pan-Akt Rabbit pAb images

ABclonal:Western blot - Pan-Akt Rabbit pAb (A3145)}

Western blot - Pan-Akt Rabbit pAb (A3145)

Western blot analysis of various lysates using Pan-Akt Rabbit pAb (A3145) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on AKT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AKT. (Distance between topics and target gene indicate popularity.) AKT

* Data provided by citexs.com, for reference only.

Publishing research using A3145? Please let us know so that we can cite the reference in this datasheet.

Antibodies (9)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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