Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Pan-Akt Rabbit mAb (A11030)

Publications (2) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Pan-Akt Rabbit mAb (A11030)

Western blot analysis of extracts of MCF-7 cells, using Pan-Akt antibody (A11030).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Pan-Akt Rabbit mAb
Catalog No. A11030
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Human AKT serine-threonine protein kinase family includes three members AKT1, AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4, 5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.

Immunogen information

Immunogen Recombinant protein of human Pan-Akt
Sequence Email for sequence
Gene ID 20720810000
Swiss prot P31749P31751Q9Y243
Synonyms AKT1/AKT2/AKT3; Pan-Akt
Calculated MW 48kDa/55kDa/51kDa/54kDa
Observed MW 56kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples MCF7
Cellular location ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle
Customer validation

WB (Homo sapiens)

Research Area

Pan-Akt Rabbit mAb images

ABclonal:Western blot - Pan-Akt Rabbit mAb (A11030)}

Western blot - Pan-Akt Rabbit mAb (A11030)

Western blot analysis of extracts of MCF-7 cells, using Pan-Akt antibody (A11030).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on AKT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AKT. (Distance between topics and target gene indicate popularity.) AKT

* Data provided by citexs.com, for reference only.

Publishing research using A11030? Please let us know so that we can cite the reference in this datasheet.

Antibodies (9)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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