Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PYGB Rabbit pAb (A13539)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PYGB Rabbit pAb (A13539)

Western blot analysis of extracts of various cell lines, using PYGB antibody (A13539) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name PYGB Rabbit pAb
Catalog No. A13539
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
Sequence LKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD
Gene ID 5834
Swiss prot P11216
Synonyms GPBB; PYGB
Calculated MW 97kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples BT-474, SW620, HepG2, Mouse lung, Rat brain
Cellular location cytoplasm, extracellular exosome, extracellular region

Documents

Certificate of Compliance

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Lot number

Research Area

PYGB Rabbit pAb images

ABclonal:Western blot - PYGB Rabbit pAb (A13539)}

Western blot - PYGB Rabbit pAb (A13539)

Western blot analysis of extracts of various cell lines, using PYGB antibody (A13539) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PYGB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PYGB. (Distance between topics and target gene indicate popularity.) PYGB

* Data provided by citexs.com, for reference only.

Publishing research using A13539? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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