Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CD45 Rabbit pAb (A0372)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CD45 Rabbit pAb (A0372)

Western blot analysis of lysates from Jurkat cells, using CD45 Rabbit pAb (A0372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - CD45 Rabbit pAb (A0372)

Immunofluorescence analysis of Jurkat cells using CD45 Rabbit pAb (A0372) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD45 Rabbit pAb (A0372)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD45 Rabbit pAb (A0372) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD45 Rabbit pAb (A0372)

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD45 Rabbit pAb (A0372) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CD45 Rabbit pAb (A0372)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg CD45 antibody (A0372). Western blot was performed from the immunoprecipitate using CD45 antibody (A0372) at a dilution of 1:1000.

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Overview

Product name CD45 Rabbit pAb
Catalog No. A0372
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 611-730 of human CD45 (NP_002829.3).
Sequence NLDEQQELVERDDEKQLMNVEPIHADILLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKEARKPFNQNKNRYVDILPYDYNRVELSEINGDAGSNYINASYIDGFKEPRKYIAAQGPRD
Gene ID 5788
Swiss prot P08575
Synonyms LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; IMD105
Calculated MW 147kDa
Observed MW 200-250kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples Jurkat
Cellular location Membrane, Membrane raft, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

CD45 Rabbit pAb images

ABclonal:Western blot - CD45 Rabbit pAb (A0372)}

Western blot - CD45 Rabbit pAb (A0372)

Western blot analysis of lysates from Jurkat cells, using CD45 Rabbit pAb (A0372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - CD45 Rabbit pAb (A0372)}

Immunofluorescence - CD45 Rabbit pAb (A0372)

Immunofluorescence analysis of Jurkat cells using CD45 Rabbit pAb (A0372) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD45 Rabbit pAb (A0372)}

Immunofluorescence - CD45 Rabbit pAb (A0372)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD45 Rabbit pAb (A0372) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD45 Rabbit pAb (A0372)}

Immunofluorescence - CD45 Rabbit pAb (A0372)

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD45 Rabbit pAb (A0372) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - CD45 Rabbit pAb (A0372)}

Immunoprecipitation - CD45 Rabbit pAb (A0372)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg CD45 antibody (A0372). Western blot was performed from the immunoprecipitate using CD45 antibody (A0372) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on PTPRC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PTPRC. (Distance between topics and target gene indicate popularity.) PTPRC

* Data provided by citexs.com, for reference only.

Publishing research using A0372? Please let us know so that we can cite the reference in this datasheet.

Antibodies (17)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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