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PTPRC Rabbit pAb (A2115)

Reviews (2) Datasheet

ABclonal:Western blot - PTPRC Polyclonal Antibody (A2115)
ABclonal:Immunohistochemistry - PTPRC Polyclonal Antibody (A2115)
ABclonal:Immunohistochemistry - PTPRC Polyclonal Antibody (A2115)
ABclonal:Immunohistochemistry - PTPRC Polyclonal Antibody (A2115)
ABclonal:Immunofluorescence - PTPRC Polyclonal Antibody (A2115)
Review
ABclonal: review for PTPRC Rabbit pAb(A2115)
Review
ABclonal: review for PTPRC Rabbit pAb(A2115)

Overview

Product namePTPRC Rabbit pAb
Catalog No.A2115
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 890-990 of human PTPRC (NP_002829.2).
SequenceRCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHPYLHNMKKRDPPSEPSPLEAEFQRLPSYRSWRTQHIGNQEENKSKNRNSNVIPYDYNRVPLKHELE
Gene ID5788
Swiss protP08575
SynonymsB220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200; PTPRC
Calculated MW130kDa/147kDa
Observed MW185kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse thymus
Cellular locationMembrane, Membrane raft, Single-pass type I membrane protein
Customer validation

(Human chronic myeloid leukemia cells)

ABclonal:Western blot - PTPRC Polyclonal Antibody (A2115) }

Western blot - PTPRC Polyclonal Antibody (A2115)

Western blot analysis of extracts of mouse thymus, using PTPRC antibody (A2115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PTPRC Polyclonal Antibody (A2115) }

Immunohistochemistry - PTPRC Polyclonal Antibody (A2115)

Immunohistochemistry of paraffin-embedded mouse lung using PTPRC antibody (A2115) (40x lens).
ABclonal:Immunohistochemistry - PTPRC Polyclonal Antibody (A2115) }

Immunohistochemistry - PTPRC Polyclonal Antibody (A2115)

Immunohistochemistry of paraffin-embedded rat kidney using PTPRC antibody (A2115) (40x lens).
ABclonal:Immunohistochemistry - PTPRC Polyclonal Antibody (A2115) }

Immunohistochemistry - PTPRC Polyclonal Antibody (A2115)

Immunohistochemistry of paraffin-embedded human esophagus using PTPRC antibody (A2115) (40x lens).
ABclonal:Immunofluorescence - PTPRC Polyclonal Antibody (A2115) }

Immunofluorescence - PTPRC Polyclonal Antibody (A2115)

Immunofluorescence analysis of Jurkat cells using PTPRC antibody (A2115) at dilution of 1:100. Blue: DAPI for nuclear staining.

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