Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Prolactin Receptor Rabbit pAb (A10513)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Prolactin Receptor Rabbit pAb (A10513)

Western blot analysis of various lysates using Prolactin Receptor Rabbit pAb (A10513) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name Prolactin Receptor Rabbit pAb
Catalog No. A10513
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 25-234 of human Prolactin Receptor (NP_000940.1).
Sequence QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMND
Gene ID 5618
Swiss prot P16471
Synonyms HPRL; MFAB; hPRLrI; RI-PRLR; Prolactin Receptor
Calculated MW 70kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, MCF7, Mouse ovary
Cellular location Membrane, Secreted, Single-pass type I membrane protein

Research Area

Prolactin Receptor Rabbit pAb images

ABclonal:Western blot - Prolactin Receptor Rabbit pAb (A10513)}

Western blot - Prolactin Receptor Rabbit pAb (A10513)

Western blot analysis of various lysates using Prolactin Receptor Rabbit pAb (A10513) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRLR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRLR. (Distance between topics and target gene indicate popularity.) PRLR

* Data provided by citexs.com, for reference only.

Publishing research using A10513? Please let us know so that we can cite the reference in this datasheet.

Proteins (1)

Antibodies (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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