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PRDM7 Polyclonal Antibody (A7961)

Datasheet

ABclonal:Western blot - PRDM7 Polyclonal Antibody (A7961)
ABclonal:Immunofluorescence - PRDM7 Polyclonal Antibody (A7961)

Overview

Product namePRDM7 Polyclonal Antibody
Catalog No.A7961
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of proteins that may have roles in transcription and other nuclear processes. The encoded protein contains a KRAB (Kruppel-associated box) domain -A box and a SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain and may function as a histone methyltransferase.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-310 of human PRDM7 (NP_001091643.1).
SequenceNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANSGYSWLITKGRNCYEYVDGKDK
Gene ID11105
Swiss protQ9NQW5
SynonymsPRDM7; PFM4; ZNF910; PR/SET domain 7
Calculated MW18kDa/45kDa/55kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, HepG2, BT-474, Jurkat, Mouse ovary, Mouse intestine, Rat ovary
Cellular locationChromosome, Nucleus
ABclonal:Western blot - PRDM7 Polyclonal Antibody (A7961) }

Western blot - PRDM7 Polyclonal Antibody (A7961)

Western blot analysis of extracts of various cell lines, using PRDM7 antibody (A7961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - PRDM7 Polyclonal Antibody (A7961) }

Immunofluorescence - PRDM7 Polyclonal Antibody (A7961)

Immunofluorescence analysis of A549 cells using PRDM7 antibody (A7961).

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