Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PPP3CC Rabbit pAb (A7714)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - PPP3CC Rabbit pAb (A7714)

Western blot analysis of extracts of various cell lines, using PPP3CC antibody (A7714) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name PPP3CC Rabbit pAb
Catalog No. A7714
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Calcineurin is a calcium-dependent, calmodulin-stimulated protein phosphatase involved in the downstream regulation of dopaminergic signal transduction. Calcineurin is composed of a regulatory subunit and a catalytic subunit. The protein encoded by this gene represents one of the regulatory subunits that has been found for calcineurin. Three transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 373-512 of human PPP3CC (NP_005596.2).
Sequence LISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS
Gene ID 5533
Swiss prot P48454
Synonyms CNA3; CALNA3; PP2Bgamma; PPP3CC
Calculated MW 58kDa
Observed MW 58kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse testis, Rat brain
Cellular location cytoplasm, cytosol, glutamatergic synapse, mitochondrion, presynapse

Documents

Certificate of Compliance

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Lot number

Research Area

PPP3CC Rabbit pAb images

ABclonal:Western blot - PPP3CC Rabbit pAb (A7714)}

Western blot - PPP3CC Rabbit pAb (A7714)

Western blot analysis of extracts of various cell lines, using PPP3CC antibody (A7714) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PPP3CC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PPP3CC. (Distance between topics and target gene indicate popularity.) PPP3CC

* Data provided by citexs.com, for reference only.

Publishing research using A7714? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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