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PPP1R12A Polyclonal Antibody (A0587)

Datasheet

ABclonal:Western blot - PPP1R12A Polyclonal Antibody (A0587)
ABclonal:Immunoprecipitation - PPP1R12A Polyclonal Antibody (A0587)

Overview

Product namePPP1R12A Polyclonal Antibody
Catalog No.A0587
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1).
SequenceMKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGG
Gene ID4659
Swiss protO14974
SynonymsPPP1R12A; M130; MBS; MYPT1; protein phosphatase 1 regulatory subunit 12A
Calculated MW105kDa/109kDa/111kDa/115kDa
Observed MW140kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesU-87MG, LO2, Jurkat, HeLa
Cellular locationCytoplasm
ABclonal:Western blot - PPP1R12A Polyclonal Antibody (A0587) }

Western blot - PPP1R12A Polyclonal Antibody (A0587)

Western blot analysis of extracts of various cell lines, using PPP1R12A antibody (A0587) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunoprecipitation - PPP1R12A Polyclonal Antibody (A0587) }

Immunoprecipitation - PPP1R12A Polyclonal Antibody (A0587)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PPP1R12A antibody (A0587). Western blot was performed from the immunoprecipitate using PPP1R12A antibody (A0587) at a dilition of 1:1000.

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