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PPAT Polyclonal Antibody (A6698)

Review (1) Datasheet

ABclonal:Western blot - PPAT Polyclonal Antibody (A6698)
ABclonal:Immunofluorescence - PPAT Polyclonal Antibody (A6698)
Review
ABclonal: review for PPAT Polyclonal Antibody(A6698)

Overview

Product namePPAT Polyclonal Antibody
Catalog No.A6698
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3).
SequenceMELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPTFKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGRLIPVSDINDKEKKTSETEGWVVSSESCSFLSIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFC
Gene ID5471
Swiss protQ06203
SynonymsPPAT; ATASE; GPAT; PRAT; amidophosphoribosyltransferase
Calculated MW57kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHepG2, COS-7
Cellular location
Customer validation

ABclonal:Western blot - PPAT Polyclonal Antibody (A6698) }

Western blot - PPAT Polyclonal Antibody (A6698)

Western blot analysis of extracts of various cell lines, using PPAT antibody (A6698) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PPAT Polyclonal Antibody (A6698) }

Immunofluorescence - PPAT Polyclonal Antibody (A6698)

Immunofluorescence analysis of HeLa cells using PPAT Polyclonal Antibody (A6698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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