Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

POLR2M Rabbit pAb (A10327)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - POLR2M Rabbit pAb (A10327)

Western blot analysis of extracts of various cell lines, using POLR2M antibody (A10327) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name POLR2M Rabbit pAb
Catalog No. A10327
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a subunit of a specific form of RNA polymerase II termed Pol II(G). The encoded protein may act as a negative regulator of transcriptional activation by the Mediator complex. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 4. Readthrough transcription between this gene and the neighboring upstream gene MYZAP (myocardial zonula adherens protein) is represented with GeneID 145781.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 73-368 of human POLR2M (NP_056347.1).
Sequence ELFNPVSLDCKLRQKAIAEVDVGTDKAQNSDPILDTSSLVPGCSSVDNIKSSQTSQNQGLGRPTLEGDEETSEVEYTVNKGPASSNRDRVPPSSEASEHHPRHRVSSQAEDTSSSFDNLFIDRLQRITIADQGEQQSEENASTKNLTGLSSGTEKKPHYMEVLEMRAKNPVPQLRKFKTNVLPFRQNDSSSHCQKSGSPISSEERRRRDKQHLDDITAARLLPLHHMPTQLLSIEESLALQKQQKQNYEEMQAKLAAQKLAERLNIKMRSYNPEGESSGRYREVRDEDDDWSSDEF
Gene ID 81488
Swiss prot P0CAP1P0CAP2Q6EEV4
Synonyms GCOM1; Gdown; Gdown1; GRINL1A; POLR2M
Calculated MW 8kDa/15kDa/24kDa/40kDa/41kDa/64kDa
Observed MW 42kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples DU145, SW620, Rat kidney
Cellular location cortical actin cytoskeleton, I band, Z disc

POLR2M Rabbit pAb images

ABclonal:Western blot - POLR2M Rabbit pAb (A10327)}

Western blot - POLR2M Rabbit pAb (A10327)

Western blot analysis of extracts of various cell lines, using POLR2M antibody (A10327) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on POLR2M. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to POLR2M. (Distance between topics and target gene indicate popularity.) POLR2M

* Data provided by citexs.com, for reference only.

Publishing research using A10327? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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