Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PMPCB Rabbit pAb (A4312)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PMPCB Rabbit pAb (A4312)

Western blot analysis of extracts of various cell lines, using PMPCB antibody (A4312) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name PMPCB Rabbit pAb
Catalog No. A4312
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 110-380 of human PMPCB (NP_004270.2).
Sequence GTKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYITTHYKGPRIVLAAAGGVSHDELLDLAKFHFGDSLCTHKGEIPALPPCKFTGSEIRVRDDKMPLAHLAIAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCHSFQSFNTSYTDTGLWGLYMVCESSTVADMLHV
Gene ID 9512
Swiss prot O75439
Synonyms MAS1; MPPB; P-52; MPP11; MPPP52; Beta-MPP; PMPCB
Calculated MW 54kDa
Observed MW 50kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples LO2, MCF7, HT-1080, Mouse liver, Mouse kidney, Mouse heart
Cellular location Mitochondrion matrix
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

PMPCB Rabbit pAb images

ABclonal:Western blot - PMPCB Rabbit pAb (A4312)}

Western blot - PMPCB Rabbit pAb (A4312)

Western blot analysis of extracts of various cell lines, using PMPCB antibody (A4312) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PMPCB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PMPCB. (Distance between topics and target gene indicate popularity.) PMPCB

* Data provided by citexs.com, for reference only.

Publishing research using A4312? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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