Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PLVAP Rabbit pAb (A15906)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - PLVAP Rabbit pAb (A15906)

Western blot analysis of various lysates using PLVAP Rabbit pAb (A15906) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name PLVAP Rabbit pAb
Catalog No. A15906
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable identical protein binding activity. Involved in MAPK cascade; positive regulation of cellular extravasation; and tumor necrosis factor-mediated signaling pathway. Located in cell surface. Colocalizes with caveola. Implicated in congenital diarrhea.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 63-442 of human PLVAP (NP_112600.1).
Sequence TERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG
Gene ID 83483
Swiss prot Q9BX97
Synonyms PV1; FELS; PV-1; gp68; DIAR10; PLVAP
Calculated MW 51kDa
Observed MW 55kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse lung, Rat lung, Rat kidney
Cellular location Cell membrane, Cytoplasm, Membrane, Single-pass type II membrane protein, caveola, perinuclear region
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

PLVAP Rabbit pAb images

ABclonal:Western blot - PLVAP Rabbit pAb (A15906)}

Western blot - PLVAP Rabbit pAb (A15906)

Western blot analysis of various lysates using PLVAP Rabbit pAb (A15906) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PLVAP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PLVAP. (Distance between topics and target gene indicate popularity.) PLVAP

* Data provided by citexs.com, for reference only.

Publishing research using A15906? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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