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PLIN2 Rabbit pAb (A6276)

Publications (3) Datasheet

ABclonal:Western blot - PLIN2 Rabbit pAb (A6276)

Western blot analysis of extracts of HepG2 cells, using PLIN2 antibody (A6276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - PLIN2 Rabbit pAb (A6276)

Western blot analysis of extracts of various cell lines, using PLIN2 antibody (A6276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - PLIN2 Rabbit pAb (A6276)

Western blot analysis of extracts of Rat heart, using PLIN2 antibody (A6276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PLIN2 Rabbit pAb (A6276)

Immunohistochemistry of paraffin-embedded rat liver using PLIN2 Rabbit pAb (A6276) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - PLIN2 Rabbit pAb (A6276)

Immunohistochemistry of paraffin-embedded human liver using PLIN2 Rabbit pAb (A6276) at dilution of 1:50 (40x lens).

ABclonal:Immunofluorescence - PLIN2 Rabbit pAb (A6276)

Immunofluorescence analysis of HeLa cells using PLIN2 Rabbit pAb (A6276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PLIN2 Rabbit pAb (A6276)

Immunofluorescence analysis of NIH-3T3 cells using PLIN2 Rabbit pAb (A6276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePLIN2 Rabbit pAb
Catalog No.A6276
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human PLIN2 (NP_001113.2).
SequenceMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH
Gene ID123
Swiss protQ99541
SynonymsPLIN2; ADFP; ADRP
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, NIH/3T3, Mouse brain, Rat heart
Cellular locationMembrane, Peripheral membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

WB(Homo sapiens)

ABclonal:Western blot - PLIN2 Rabbit pAb (A6276) }

Western blot - PLIN2 Rabbit pAb (A6276)

Western blot analysis of extracts of HepG2 cells, using PLIN2 antibody (A6276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - PLIN2 Rabbit pAb (A6276) }

Western blot - PLIN2 Rabbit pAb (A6276)

Western blot analysis of extracts of various cell lines, using PLIN2 antibody (A6276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - PLIN2 Rabbit pAb (A6276) }

Western blot - PLIN2 Rabbit pAb (A6276)

Western blot analysis of extracts of Rat heart, using PLIN2 antibody (A6276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PLIN2 Rabbit pAb (A6276) }

Immunohistochemistry - PLIN2 Rabbit pAb (A6276)

Immunohistochemistry of paraffin-embedded rat liver using PLIN2 Rabbit pAb (A6276) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - PLIN2 Rabbit pAb (A6276) }

Immunohistochemistry - PLIN2 Rabbit pAb (A6276)

Immunohistochemistry of paraffin-embedded human liver using PLIN2 Rabbit pAb (A6276) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - PLIN2 Rabbit pAb (A6276) }

Immunofluorescence - PLIN2 Rabbit pAb (A6276)

Immunofluorescence analysis of HeLa cells using PLIN2 Rabbit pAb (A6276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PLIN2 Rabbit pAb (A6276) }

Immunofluorescence - PLIN2 Rabbit pAb (A6276)

Immunofluorescence analysis of NIH-3T3 cells using PLIN2 Rabbit pAb (A6276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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