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PLCG2 Polyclonal Antibody (A2182)

Datasheet

ABclonal:Western blot - PLCG2 Polyclonal Antibody (A2182)
ABclonal:Immunohistochemistry - PLCG2 Polyclonal Antibody (A2182)
ABclonal:Immunofluorescence - PLCG2 Polyclonal Antibody (A2182)

Overview

Product namePLCG2 Polyclonal Antibody
Catalog No.A2182
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PLCG2 (NP_002652.2).
SequenceMSTTVNVDSLAEYEKSQIKRALELGTVMTVFSFRKSTPERRTVQVIMETRQVAWSKTADKIEGFLDIMEIKEIRPGKNSKDFERAKAVRQKEDCCFTILYGTQFVLSTLSLAADSKEDAVNWLSGLKILHQEAMNASTPTIIESWLRKQIYSVDQTRRNSISLRELKTILPLINFKVSSAKFLKDKFVEIGAHKDELSFEQFHLFYKKLMFEQQKSILDEFKKDSSVFILGNTDRPDASAVYLHDFQRFLIHEQQEHWAQDLNKVRERMTKFIDDTMRETAEPFLFVDEFLTYLFSRENS
Gene ID5336
Swiss protP16885
SynonymsPLCG2; APLAID; FCAS3; PLC-IV; PLC-gamma-2; phospholipase C gamma 2
Calculated MW147kDa
Observed MW175kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, Raji, Ramos, HeLa
Cellular location
ABclonal:Western blot - PLCG2 Polyclonal Antibody (A2182) }

Western blot - PLCG2 Polyclonal Antibody (A2182)

Western blot analysis of extracts of various cell lines, using PLCG2 antibody (A2182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - PLCG2 Polyclonal Antibody (A2182) }

Immunohistochemistry - PLCG2 Polyclonal Antibody (A2182)

Immunohistochemistry of paraffin-embedded mouse heart using PLCG2 antibody (A2182) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - PLCG2 Polyclonal Antibody (A2182) }

Immunofluorescence - PLCG2 Polyclonal Antibody (A2182)

Immunofluorescence analysis of U2OS cells using PLCG2 antibody (A2182).

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