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PI3 Kinase p85 alpha Rabbit pAb (A11177)

Reviews (3)Publications (2) Datasheet

ABclonal:Western blot - PI3 Kinase p85 alpha Polyclonal Antibody (A11177)
Review
ABclonal: review for PI3 Kinase p85 alpha Rabbit pAb(A11177)
Review
ABclonal: review for PI3 Kinase p85 alpha Rabbit pAb(A11177)
Review
ABclonal: review for PI3 Kinase p85 alpha Rabbit pAb(A11177)

Overview

Product namePI3 Kinase p85 alpha Rabbit pAb
Catalog No.A11177
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852556.2).
SequenceEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRE
Gene ID5295
Swiss protP27986
SynonymsAGM7; GRB1; IMD36; p85; p85-ALPHA; PI3 Kinase p85 alpha; PIK3R1
Calculated MW42kDa/49kDa/53kDa/83kDa/84kDa
Observed MW85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-251MG, Jurkat, SKOV3, mouse brain, mouse liver, rat brain, rat liver
Cellular location
Customer validation

(Human chronic myeloid leukemia cells)

WB(Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - PI3 Kinase p85 alpha Polyclonal Antibody (A11177) }

Western blot - PI3 Kinase p85 alpha Polyclonal Antibody (A11177)

Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 alpha antibody (A11177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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