Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PHLPP1 Rabbit pAb (A9542)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PHLPP1 Rabbit pAb (A9542)

Western blot analysis of extracts of various cell lines, using PHLPP1 antibody (A9542) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - PHLPP1 Rabbit pAb (A9542)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PHLPP1 Rabbit pAb (A9542) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PHLPP1 Rabbit pAb (A9542)

Immunofluorescence analysis of U-2 OS cells using PHLPP1 Polyclonal Antibody (A9542) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name PHLPP1 Rabbit pAb
Catalog No. A9542
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1160-1428 of human PHLPP1 (NP_919431.2).
Sequence DQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDS
Gene ID 23239
Swiss prot O60346
Synonyms SCOP; PHLPP; PPM3A; PLEKHE1; PHLPP1
Calculated MW 185kDa
Observed MW 185kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, SH-SY5Y, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular location Cell membrane, Cytoplasm, Membrane, Nucleus, Peripheral membrane protein

Documents

Certificate of Compliance

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Research Area

PHLPP1 Rabbit pAb images

ABclonal:Western blot - PHLPP1 Rabbit pAb (A9542)}

Western blot - PHLPP1 Rabbit pAb (A9542)

Western blot analysis of extracts of various cell lines, using PHLPP1 antibody (A9542) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - PHLPP1 Rabbit pAb (A9542)}

Immunohistochemistry - PHLPP1 Rabbit pAb (A9542)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PHLPP1 Rabbit pAb (A9542) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PHLPP1 Rabbit pAb (A9542)}

Immunofluorescence - PHLPP1 Rabbit pAb (A9542)

Immunofluorescence analysis of U-2 OS cells using PHLPP1 Polyclonal Antibody (A9542) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PHLPP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PHLPP1. (Distance between topics and target gene indicate popularity.) PHLPP1

* Data provided by citexs.com, for reference only.

Publishing research using A9542? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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