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PHLDA2 Polyclonal Antibody (A6244)

Datasheet

ABclonal:Western blot - PHLDA2 Polyclonal Antibody (A6244)
ABclonal:Immunofluorescence - PHLDA2 Polyclonal Antibody (A6244)

Overview

Product namePHLDA2 Polyclonal Antibody
Catalog No.A6244
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human PHLDA2 (NP_003302.1).
SequenceMKSPDEVLREGELEKRSDSLFQLWKKKRGVLTSDRLSLFPASPRARPKELRFHSILKVDCVERTGKYVYFTIVTTDHKEIDFRCAGESCWNAAIALALIDFQNRRALQDFRSRQERTAPAAPAEDAVAAAAAAPSEPSEPSRPSPQPKPRTP
Gene ID7262
Swiss protQ53GA4
SynonymsPHLDA2; BRW1C; BWR1C; HLDA2; IPL; TSSC3
Calculated MW17kDa
Observed MW20kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesSW620, SKOV3, HeLa
Cellular locationCytoplasm, Membrane, Peripheral membrane protein
ABclonal:Western blot - PHLDA2 Polyclonal Antibody (A6244) }

Western blot - PHLDA2 Polyclonal Antibody (A6244)

Western blot analysis of extracts of various cell lines, using PHLDA2 antibody (A6244) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - PHLDA2 Polyclonal Antibody (A6244) }

Immunofluorescence - PHLDA2 Polyclonal Antibody (A6244)

Immunofluorescence analysis of HeLa cells using PHLDA2 antibody (A6244). Blue: DAPI for nuclear staining.

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